Dr.Madiha Khalid

Email: madiha@hsa.edu.pk

ORCID ID:
0000-0001-7647-354X |

RESEARCH GATE
PROFILE:
https://www.researchgate.net/pro
file/Madiha-Khalid-11

SKILLS
 Molecular cloning
 RNA & and DNA
extraction (Tissue and
cells)
 qRT- PCR to study gene
expression.
 ELISA
 IHC &
Immunofluorescence
 SDS-PAGE & Western
Blot
 Maintenance of cell lines
(BSL-2, BSL-3)
 Adherent and suspension
cultures
 Transfection methods
 In vitro infectivity
 TCID50 (ISO:18184)
 Plaque Assay
 Cell sorting using FACS
 Antibacterial assays
(ISO:20743)
 Transwell migration assay
 Flex Station 3 Multi-Mode
Micro plate Reader
 Incucyte Live-Cell
analysis system
 Cytotoxicity assay
 MTT assay

Dr. Madiha Khalid

Academic Qualification

Professional Experience

RESEARCH STUDENTS (CURRENT/GRADUATED)

M. Phil: 2/12, BS: 3/10
Journal Publications
1. “Development of protective cotton textiles against biohazards and harmful UV radiation
using eco-friendly novel fiber-reactive bioactive agent (2022). A Nosheen, MT Hussain,
M Khalid, A Javid, H Aziz, S Iqbal, M Ashraf, Process Safety and Environmental
Protection. IF=7.91; ISSN: 1744-3598,0957-5820

2. P2X4 receptors mediate induction of antioxidants, fibrogenic cytokines and ECM
transcripts; in presence of replicating HCV in vitro setting: An insight into role of fibrosis
(2022). S Manzoor, M Khalid, M Idrees PloS one; IF=3.58; ISSN: 1932-6203 3.58.

3. Role of viral and host factors in interferon based therapy of hepatitis C virus infection
(2014) M Imran, S Manzoor, J Ashraf, M Khalid, M Tariq, HM Khaliq, S Azam.
Virology Journal. 2014, IF=2.55; ISSN:1743-422X

4. Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV
hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor (2018).
M Khalid, S Manzoor, H Ahmad, A Asif, TA Bangash, A Latif, S Jaleel Molecular
biology reports. IF=2.32; ISSN: 0301-4851,1573-4978

5. Alocasia odora mediated synthesis of silver nanoparticles, their cytotoxicity and virucidal
potential” (2022). Humaira Naureen; Rabia Khan; Aneela Javed; Madiha Khalid; Hina
Khan. Applied Microbiology and Biotechnology. IF=3.53; ISSN:1432-0614,0175-7598

6. Pilot-scale production of highly durable bioactive and UV-protective cotton fabric by
electroless deposition of copper on cotton fabric (2022). Anum Nosheen, Madiha
Khalid, Sobia Manzoor, Munir Ashraf, Zhebin Xue, Daniyal Sajid Khan, Sidra Urooj,
Asraf Hussain Hashmi| Cellulose| IF=6.20; ISSN: 0969-0239

7. Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: An
approach towards proinflammatory role of P2X4 and P2X7 receptors (2019). A Asif, M
Khalid, S Manzoor, H Ahmad, AU Rehman Purinergic signaling. IF= 2.635;
ISSN:1573-9538,1573-954.

8. Erratum to: Current and future therapies for hepatitis C virus infection: from viral proteins
to host targets SAMA (2014). Muhammad Imran, Sobia Manzoor, Nasir Mahmood
Khattak, Madiha Khalid Archives of Virology. IF-2.57; ISSN: 0304-8608,1432-8798

9. Hepatitis C virus infection in vitro triggers endoplasmic reticulum stress and
downregulates insulin receptor substrates 1 and 2 through upregulation of cytokine
signaling. (2014). QL Ahmed, S Manzoor, M Tariq, M Khalid, W Ashraf, F Parvaiz, M
Imran Acta Virol IF=1.22;ISSN: 1336-2305,0001-723X

10. Carcinoma-specific expression of P2Y11 receptor and its contribution in ATP-induced
purinergic signalling and cell migration in human hepatocellular carcinoma cells (2017).
M Khalid, L Brisson, M Tariq, Y Hao, R Guibon, G Fromont, SAS Mortadza.
Oncotarget. IF=3.66; ISSN=1949-2553

11. Zika virus: immune evasion mechanisms, currently available therapeutic regimens, and
vaccines (2017). A Asif, S Manzoor, F Tuz-Zahra, M Saalim, M Ashraf, J Ishtiyaq, M
Khalid Viral immunology. IF= 2.175; ISSN:1557-8976,0882-8245

12. Ionotropic purinergic receptors P2X4 and P2X7: proviral or antiviral? An insight into
P2X receptor signaling and hepatitis C virus infection. (2016) S Manzoor, U Akhtar, S
Naseem, M Khalid, M Mazhar, F Parvaiz, S Khaliq. Viral Immunology. IF= 2.175;
ISSN: 1557- 8976,0882-8245.

13. Correlation of OAS1 gene polymorphism at exon 7 splice accepter site with interferonbased therapy of HCV infection in Pakistan. (2014) M Imran, S Manzoor, NM Khattak,
M Tariq, M Khalid, F Javed, S Bhatti. Viral Immunology. IF=2.175; ISSN: 1557-
8976,0882-8245

14. Transcript analysis of P2X receptors in PBMCs of chronic HCV patients: an insight
into antiviral treatment response and HCV-induced pathogenesis. (2013) W Ashraf, S
Manzoor, J Ashraf, QL Ahmed, M Khalid, M Tariq, M Imran. Viral Immunology.
IF=2.175; ISSN: 1557- 8976,0882-8245.

15. NOX4 induces oxidative stress and apoptosis through upregulation of caspases 3 and 9
and downregulation of TIGAR in HCV-infected Huh-7 cells. (2013) M Tariq, S
Manzoor, QL Ahmed, M Khalid, W Ashraf. Future Virology. IF=3.01; ISSN: 1746-
0794

16. Investigation of biomarkers in peripheral blood cells of Pakistani chronic HCV patients
with genotype 3a: an assessment of HCV-induced pathogenesis. (2014) M Khalid, S
Manzoor, M Tariq, M Imran, W Ashraf, QL Ahmed, F Javed Future Virology.
IF=3.01; ISSN: 1746-0794

17. Development of murine models to study Hepatitis C virus induced liver pathogenesis.
(2013) M Khalid, S Manzoor, M Imran, M Tariq, J Ashraf, QL Ahmed, W
Ashraf.Indian Journal of Virology. IF=0.98; ISSNN:

18. Khalid M, Nazar, M. S, Hafeez, U, Haq, I. ul, Khalid, S., & Zakki, S. A. (2023).
Structural Evaluation of Interaction between HCV Enveloped Protein E1 and P2X4
Receptor on Hepatocytes. Avicenna Journal of Medical Sciences, 1(2); ISSN: 2958-
2741

19. Khalid, R. ., Subhan Nazar, M. ., Ahmad Zakki, S. ., Altaf, E. ., Raza, A. ., Saeed,
M.and Khalid, M. (2023) “Impact of Poverty on Secondary School Academic
Achievement among Girls in District Faisalabad, Pakistan; A cross sectional study:
Impact of Poverty on secondary school students”, Pakistan BioMedical Journal, 6(02),
pp. 12–16; ISSN: 2709-278X

20. Khalid, R., Inam, A., Nazar, M. S., Zakki, S. A., Altaf, E., & Khalid, M. (2023).
Assessment and Treatment Therapies of Stammering used by Speech-Language
Pathologists, A cross sectional study. Avicenna Journal of Medical Sciences, 1(2)

ISSN: 2958-2741
Journal Reviewer
Editorial Services
References

DISEASE MARKER
WORLD JOURNAL OF BIOLOGY AND BIOTECHNOLOGY
PLANT HEALTH
BIOSIGHT JOURNAL
Academic Editor for specialty section “Gastroenterology”
Journal: Advances in Medicine
1. Dr. Hussnain Ali Janjua
HOD, Industrial Biotechnology
ASAB, National University of Sciences and Technology (NUST).H-12,
Islamabad
Email: janjuahussnain@gmail.com

2. Dr. Habib Ahmed
HOD, Air University School of Management-
(AUSOM)
Air University, Islamabad
Email: habib.ahmed@mail.au.edu.p

Video Presentations

  1. Policy presentation (video format) in titled “Tobacco Harm Reduction Can Help Pakistan Successfully Fight Smoking Epidemic” has been published in GFN Fives at Marriott Hotel in Warsaw Poland. https://gfn.events/videos/118/tobacco-harm-reduction-can-help-pakistan-successfully-fight-smoking-epidemic

Radio Programs on Smoking Cessation (Radio Pakistan)

http://www.aripk.com/radio-programs.php

  • Program No. 2
  • Program No. 6
  • Program No. 10

Work as a Reviewer

  1. PLOS ONE (Impact Factor 3.24)

https://journals.plos.org/plosone/static/publish

  1. Frontiers in Public Health (Impact Factor 3.71)

https://www.frontiersin.org/journals/public-health

  1. Cities (Impact Factor 3.85)

https://www.journals.elsevier.com/cities

  1. Agricultural and Resource Economics: International Scientific E-Journal (Scopus and Web of Science)

https://are-journal.com/are

  1. Health Education Research (Impact Factor 2.22)

https://academic.oup.com/her

  1. Sustainable Development (Impact Factor 12.5)

https://onlinelibrary.wiley.com/journal/10991719

  1. Sage Open (Impact Factor 2.0)

https://journals.sagepub.com/home/sgo

  1. International Journal of Agricultural Sustainability (Impact Factor 3.4)

https://www.tandfonline.com/journals/tags20

  1. Tobacco Use Insights (Impact Factor 2.2)

https://journals.sagepub.com/home/tuia

Received Scholarships

  1. Prime Minister’s Fee Reimbursement Scheme for Less Developed Areas 2015-16 (For PhD)
  1. Tobacco Harm Reduction Scholarship-2023 by Knowledge, Action, Change, London, UK (PKR 3.8 Million)

Language Skills (indicate only languages in which you can work): English, Urdu

Completed Projects:

Reference to Prior Work/Assignments that

Best Illustrates Capability to Handle the

Assigned Tasks

1.     BISP NSER Update Third Party Operations Review

Client: BISP

Coverage: Overall Pakistan (156 districts, including 14 pilot districts)

Date: June 2018- date

Role assigned: Senior Economic Analyst

Responsibilities performed: Developed NSER census monitoring and evaluation plan, strategies, sampling design, instruments, coverage, and content error design. Train master trainers and field staff for M&E activities and spot check survey. Performed robust data analysis for the coverage and content errors.

 

2.     Clinical Study Protocol on Electronic Cigarettes and Nicotine Pouches for Smoking Cessation in Pakistan: A Randomized Controlled Trial

Client: ARI

Coverage: Islamabad, Rawalpindi

Date: Feb -2023 to Jun 2023

Role: Senior Researcher

Responsibilities: Developed survey questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Perform quantitative and qualitative data analysis and report writing.

 

3.     Situational Analysis of SDG 1 Localization in Sindh Province of Pakistan

Client: Momentum Ventures (Pvt.) Ltd, Sindh Govt, SDGs Unit, UNICEF and UNDP

Coverage: Province Sindh

Date: December 2022 to March 2023

Role: Poverty Sector Expert

Responsibilities: Review poverty data, calculate poverty statistics, estimate MPI and population below poverty line after floods, guide budget allocations for pro-poor programs, update estimates for poverty eradication, participate in consultations, suggest data consolidation mechanisms, and prepare policy recommendations for localizing SDG 1 in Sindh.

 

 

4.     National Program for Enhancing the Command Area in Barani Areas of Pakistan

Client: Government of Pakistan

Coverage: Overall Pakistan (Excluding Sindh Province)

Date: September 2022- November 2022

Role assigned: Senior Monitoring and Evaluation Expert

Responsibilities performed: Developed monitoring and evaluation, and impact evaluation plan, strategies, sampling design and instruments. Train master trainers and field staff for spot-check activities and spot-check surveys. Perform statistical analysis for the monitoring and evaluation and Impact evaluation.

 

5.     Knowledge towards Harm Reduction and Nicotine among Public Health Practitioners in Pakistan

Client: ARI

Coverage: Islamabad, Rawalpindi

Date: Feb -2022 to Jan 2023

Role: Senior Researcher

Responsibilities: Developed survey questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Perform quantitative and qualitative data analysis and report writing.

6.     Baseline Survey for Global Climate Fund (GCF) Project “Transforming the Indus Basin with Climate Resilient Agriculture and Water Management

Client: FAO/Momentum Ventures

Coverage: Dera Ghazi Khan, Khanewal, Lodhran, Multan, Muzaffargarh, Badin, Sanghar, and Umerkot

Date: April 2022- July 2022

Role: Team Leader

Responsibilities: Manage and control project activities, developed survey tools and questionnaires, survey-sampling design. Train enumerators for survey activities. Manage quantitative and qualitative data analysis and report writing and editing.

 

7.     Assessing the Knowledge, Attitude and Practices of Cigarette Smokers and Use of Alternative Nicotine Delivery Systems in Pakistan: A Cross-Sectional Study

Client: ARI

Coverage: Islamabad, Lahore, Multan, Muzaffargarh, Hyderabad, Karachi, Nawabshah, Peshawar, Swabi, Quetta, Qila Abdullah, Bagh, Ghanche

Date: April 2020- Feb 2020

Role assigned: Senior Research Analyst

Responsibilities performed: Developed KAP questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Performed quantitative and qualitative data analysis and report writing.

8.     Rapid Assessment of Smoking and Vaping in Pakistan during COVID-19 Lockdown

Client: ARI

Coverage: Islamabad, Rawalpindi, Lahore, Faisalabad, Sialkot, Multan, Peshawar, Abbottabad, Quetta, Karachi, and Hyderabad

Date: Jan 2020- May 2020

Role assigned: Senior Research Analyst

Responsibilities performed: Developed semi-structure questionnaire, survey implementation plan and sampling design. Train enumerators for telephonic survey activities. Performed quantitative and qualitative data analysis and report writing.

9.     Knowledge, Attitude and Practice (KAP) Study on Vapers in Pakistan

Client: ARI

Coverage: Islamabad, Rawalpindi, Sialkot, Lahore, Faisalabad, Multan, Hyderabad, Karachi, Peshawar, Abbottabad, and Quetta

Date: July 2019- December 2019

Role assigned: Senior Research Analyst

Responsibilities performed: Developed KAP questionnaire, survey implementation plan and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed quantitative and qualitative data analysis and report writing.

10.   Barriers to Smoking Cessation in Marginalized Communities and the Use of Harm reduction Product in Islamabad, Pakistan

Client: ARI

Coverage: Marginalized Area of Islamabad

Date: Jan 2019- Jun 2019

Role assigned: Senior Research Analyst

Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative data analysis and report writing.

11.  Socio-economic Profile of Out-of-School Children 5-16 Years of Age in Pakistan: A Preliminary Analysis

Client: Pakistan Coalition for Education

Coverage: Secondary Research from 2014-2019 across Pakistan

Date: Jan- June 2020

Role assigned: Research Analyst

Responsibilities performed: Performed Secondary data analysis and statistical report writing.

12.  Rapid Assessment of Psychological Effect of COVID-19 & School closure on Children

Client: Pakistan Coalition for Education

Coverage: Islamabad, Rawalpindi, Karachi, Peshawar, Quetta, Muzaffarabad, and Gilgit

Date: Jun 2020- December 2020

Role assigned: Research Analyst

Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative and quantitative data analysis.

13.  Third Party Verification of Waseela-e-Rozgar Liabilities

Client: BISP

Coverage: 77 districts (20 Service providers and 213 training institutes)

Date: Dec 2019- April 2020

Role assigned: Senior Economic Analyst

Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative data analysis and report writing.

14.  Multi-topic Targeting Performance Survey

Client: BISP/The World Bank

Coverage: 4 districts

Date: March –June 2017

Role assigned: Economic Analyst

Responsibilities performed: Developed survey instruments, developed sample design, delivered data collection training, supervised district teams. Performed data analysis

15.  Youth Workforce Development Project (YWFD) Evaluation 

Client: MSI/USAID:

Coverage:  Karachi

Dates: Aug 2016-Oct 2016

Role assigned: Economic Analyst

Responsibilities performed: Data Management and Analysis

16.  Humqadam School Construction & Rehabilitation Programme: Baseline Assessment

Client: IMC Worldwide:

Coverage:  7 districts

Dates: Apr 2016-Jul 2016

Role assigned: Economic Analyst

Responsibilities performed: Data Management and Analysis

17.  Operational Review of the Waseela-e-Taleem Programme

Client: BISP/ The World Bank

Coverage: 13 districts

Dates: April 2013- June 2017

Role assigned: Economic Analyst

Responsibilities performed: Delivered data collection training to enumerators and supervisors, developed sample design, developed structural questionnaire, carried out data analysis

18.  Monitoring of the National Household Survey of Social Protection and Cohesion in Pakistan

Client: The World Bank

Coverage:  90 cluster across Pakistan

Date: Nov 2015 – March 2016

Role assigned: Economic Analyst, Field Supervisor

Responsibilities performed: Monitoring of data collection activities, secondary sampling unit selection, carried out data cleaning, coding and analysis

19.  Pakistan Household Skills Measurement Survey (Wave II & III)

Client:  The World Bank

Coverage: 17 districts

Date 2014 –2015

Role assigned: Research Assistant

Responsibilities performed: Data cleaning, coding, and analysis

20.  Punjab Skills Development Fund Furniture Manufacturing Sector Skill Study

Client: Punjab Skill Development Fund (Govt. Of Pakistan)

Coverage: 4 districts

Date: April – November 2015

Role assigned: Research Assistant

Responsibilities performed: Sampling design, data cleaning, coding, and data analysis

21.  The Agriculture Drip Irrigation Discrete Choice Experiment Survey

Client: IFPRI

Coverage: 4 districts

Date: Jan 2015-Jun 2015

Role assigned: Research Assistant

Responsibilities performed: Data Management, data cleaning, coding, and data analysis

 

22.  Baseline Survey for Income Generation project Phase I

Client: World Vision

Coverage: 5 districts

Date: 2015

Role assigned: Research Assistant

Responsibilities performed: Sampling design, data cleaning, coding, and analysis

23.  Pakistan Panel Rural Household Survey

Client: IFPRI

Coverage: 19 districts

Date: 2012 – 2015

Role assigned: Research Assistant

Responsibilities performed: Data cleaning, coding, and analysis

24.  Pakistan Agriculture Production Survey

Client: IFPRI

Coverage: 19 districts

Date: 2014 – 2015

Role assigned: Research Assistant, Field supervisor 

Responsibilities performed: Data cleaning, coding, and analysis