Email: madiha@hsa.edu.pk
ORCID ID:
0000-0001-7647-354X |
RESEARCH GATE
PROFILE:
https://www.researchgate.net/pro
file/Madiha-Khalid-11
SKILLS
Molecular cloning
RNA & and DNA
extraction (Tissue and
cells)
qRT- PCR to study gene
expression.
ELISA
IHC &
Immunofluorescence
SDS-PAGE & Western
Blot
Maintenance of cell lines
(BSL-2, BSL-3)
Adherent and suspension
cultures
Transfection methods
In vitro infectivity
TCID50 (ISO:18184)
Plaque Assay
Cell sorting using FACS
Antibacterial assays
(ISO:20743)
Transwell migration assay
Flex Station 3 Multi-Mode
Micro plate Reader
Incucyte Live-Cell
analysis system
Cytotoxicity assay
MTT assay
RESEARCH STUDENTS (CURRENT/GRADUATED)
M. Phil: 2/12, BS: 3/10
Journal Publications
1. “Development of protective cotton textiles against biohazards and harmful UV radiation
using eco-friendly novel fiber-reactive bioactive agent (2022). A Nosheen, MT Hussain,
M Khalid, A Javid, H Aziz, S Iqbal, M Ashraf, Process Safety and Environmental
Protection. IF=7.91; ISSN: 1744-3598,0957-5820
2. P2X4 receptors mediate induction of antioxidants, fibrogenic cytokines and ECM
transcripts; in presence of replicating HCV in vitro setting: An insight into role of fibrosis
(2022). S Manzoor, M Khalid, M Idrees PloS one; IF=3.58; ISSN: 1932-6203 3.58.
3. Role of viral and host factors in interferon based therapy of hepatitis C virus infection
(2014) M Imran, S Manzoor, J Ashraf, M Khalid, M Tariq, HM Khaliq, S Azam.
Virology Journal. 2014, IF=2.55; ISSN:1743-422X
4. Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV
hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor (2018).
M Khalid, S Manzoor, H Ahmad, A Asif, TA Bangash, A Latif, S Jaleel Molecular
biology reports. IF=2.32; ISSN: 0301-4851,1573-4978
5. Alocasia odora mediated synthesis of silver nanoparticles, their cytotoxicity and virucidal
potential” (2022). Humaira Naureen; Rabia Khan; Aneela Javed; Madiha Khalid; Hina
Khan. Applied Microbiology and Biotechnology. IF=3.53; ISSN:1432-0614,0175-7598
6. Pilot-scale production of highly durable bioactive and UV-protective cotton fabric by
electroless deposition of copper on cotton fabric (2022). Anum Nosheen, Madiha
Khalid, Sobia Manzoor, Munir Ashraf, Zhebin Xue, Daniyal Sajid Khan, Sidra Urooj,
Asraf Hussain Hashmi| Cellulose| IF=6.20; ISSN: 0969-0239
7. Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: An
approach towards proinflammatory role of P2X4 and P2X7 receptors (2019). A Asif, M
Khalid, S Manzoor, H Ahmad, AU Rehman Purinergic signaling. IF= 2.635;
ISSN:1573-9538,1573-954.
8. Erratum to: Current and future therapies for hepatitis C virus infection: from viral proteins
to host targets SAMA (2014). Muhammad Imran, Sobia Manzoor, Nasir Mahmood
Khattak, Madiha Khalid Archives of Virology. IF-2.57; ISSN: 0304-8608,1432-8798
9. Hepatitis C virus infection in vitro triggers endoplasmic reticulum stress and
downregulates insulin receptor substrates 1 and 2 through upregulation of cytokine
signaling. (2014). QL Ahmed, S Manzoor, M Tariq, M Khalid, W Ashraf, F Parvaiz, M
Imran Acta Virol IF=1.22;ISSN: 1336-2305,0001-723X
10. Carcinoma-specific expression of P2Y11 receptor and its contribution in ATP-induced
purinergic signalling and cell migration in human hepatocellular carcinoma cells (2017).
M Khalid, L Brisson, M Tariq, Y Hao, R Guibon, G Fromont, SAS Mortadza.
Oncotarget. IF=3.66; ISSN=1949-2553
11. Zika virus: immune evasion mechanisms, currently available therapeutic regimens, and
vaccines (2017). A Asif, S Manzoor, F Tuz-Zahra, M Saalim, M Ashraf, J Ishtiyaq, M
Khalid Viral immunology. IF= 2.175; ISSN:1557-8976,0882-8245
12. Ionotropic purinergic receptors P2X4 and P2X7: proviral or antiviral? An insight into
P2X receptor signaling and hepatitis C virus infection. (2016) S Manzoor, U Akhtar, S
Naseem, M Khalid, M Mazhar, F Parvaiz, S Khaliq. Viral Immunology. IF= 2.175;
ISSN: 1557- 8976,0882-8245.
13. Correlation of OAS1 gene polymorphism at exon 7 splice accepter site with interferonbased therapy of HCV infection in Pakistan. (2014) M Imran, S Manzoor, NM Khattak,
M Tariq, M Khalid, F Javed, S Bhatti. Viral Immunology. IF=2.175; ISSN: 1557-
8976,0882-8245
14. Transcript analysis of P2X receptors in PBMCs of chronic HCV patients: an insight
into antiviral treatment response and HCV-induced pathogenesis. (2013) W Ashraf, S
Manzoor, J Ashraf, QL Ahmed, M Khalid, M Tariq, M Imran. Viral Immunology.
IF=2.175; ISSN: 1557- 8976,0882-8245.
15. NOX4 induces oxidative stress and apoptosis through upregulation of caspases 3 and 9
and downregulation of TIGAR in HCV-infected Huh-7 cells. (2013) M Tariq, S
Manzoor, QL Ahmed, M Khalid, W Ashraf. Future Virology. IF=3.01; ISSN: 1746-
0794
16. Investigation of biomarkers in peripheral blood cells of Pakistani chronic HCV patients
with genotype 3a: an assessment of HCV-induced pathogenesis. (2014) M Khalid, S
Manzoor, M Tariq, M Imran, W Ashraf, QL Ahmed, F Javed Future Virology.
IF=3.01; ISSN: 1746-0794
17. Development of murine models to study Hepatitis C virus induced liver pathogenesis.
(2013) M Khalid, S Manzoor, M Imran, M Tariq, J Ashraf, QL Ahmed, W
Ashraf.Indian Journal of Virology. IF=0.98; ISSNN:
18. Khalid M, Nazar, M. S, Hafeez, U, Haq, I. ul, Khalid, S., & Zakki, S. A. (2023).
Structural Evaluation of Interaction between HCV Enveloped Protein E1 and P2X4
Receptor on Hepatocytes. Avicenna Journal of Medical Sciences, 1(2); ISSN: 2958-
2741
19. Khalid, R. ., Subhan Nazar, M. ., Ahmad Zakki, S. ., Altaf, E. ., Raza, A. ., Saeed,
M.and Khalid, M. (2023) “Impact of Poverty on Secondary School Academic
Achievement among Girls in District Faisalabad, Pakistan; A cross sectional study:
Impact of Poverty on secondary school students”, Pakistan BioMedical Journal, 6(02),
pp. 12–16; ISSN: 2709-278X
20. Khalid, R., Inam, A., Nazar, M. S., Zakki, S. A., Altaf, E., & Khalid, M. (2023).
Assessment and Treatment Therapies of Stammering used by Speech-Language
Pathologists, A cross sectional study. Avicenna Journal of Medical Sciences, 1(2)
ISSN: 2958-2741
Journal Reviewer
Editorial Services
References
DISEASE MARKER
WORLD JOURNAL OF BIOLOGY AND BIOTECHNOLOGY
PLANT HEALTH
BIOSIGHT JOURNAL
Academic Editor for specialty section “Gastroenterology”
Journal: Advances in Medicine
1. Dr. Hussnain Ali Janjua
HOD, Industrial Biotechnology
ASAB, National University of Sciences and Technology (NUST).H-12,
Islamabad
Email: janjuahussnain@gmail.com
2. Dr. Habib Ahmed
HOD, Air University School of Management-
(AUSOM)
Air University, Islamabad
Email: habib.ahmed@mail.au.edu.p
Video Presentations
Radio Programs on Smoking Cessation (Radio Pakistan)
http://www.aripk.com/radio-programs.php
Work as a Reviewer
https://journals.plos.org/plosone/static/publish
https://www.frontiersin.org/journals/public-health
https://www.journals.elsevier.com/cities
https://onlinelibrary.wiley.com/journal/10991719
https://journals.sagepub.com/home/sgo
https://www.tandfonline.com/journals/tags20
https://journals.sagepub.com/home/tuia
Received Scholarships
Language Skills (indicate only languages in which you can work): English, Urdu
Reference to Prior Work/Assignments that Best Illustrates Capability to Handle the Assigned Tasks |
1. BISP NSER Update Third Party Operations Review Client: BISP Coverage: Overall Pakistan (156 districts, including 14 pilot districts) Date: June 2018- date Role assigned: Senior Economic Analyst Responsibilities performed: Developed NSER census monitoring and evaluation plan, strategies, sampling design, instruments, coverage, and content error design. Train master trainers and field staff for M&E activities and spot check survey. Performed robust data analysis for the coverage and content errors.
2. Clinical Study Protocol on Electronic Cigarettes and Nicotine Pouches for Smoking Cessation in Pakistan: A Randomized Controlled Trial Client: ARI Coverage: Islamabad, Rawalpindi Date: Feb -2023 to Jun 2023 Role: Senior Researcher Responsibilities: Developed survey questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Perform quantitative and qualitative data analysis and report writing.
3. Situational Analysis of SDG 1 Localization in Sindh Province of Pakistan Client: Momentum Ventures (Pvt.) Ltd, Sindh Govt, SDGs Unit, UNICEF and UNDP Coverage: Province Sindh Date: December 2022 to March 2023 Role: Poverty Sector Expert Responsibilities: Review poverty data, calculate poverty statistics, estimate MPI and population below poverty line after floods, guide budget allocations for pro-poor programs, update estimates for poverty eradication, participate in consultations, suggest data consolidation mechanisms, and prepare policy recommendations for localizing SDG 1 in Sindh.
4. National Program for Enhancing the Command Area in Barani Areas of Pakistan Client: Government of Pakistan Coverage: Overall Pakistan (Excluding Sindh Province) Date: September 2022- November 2022 Role assigned: Senior Monitoring and Evaluation Expert Responsibilities performed: Developed monitoring and evaluation, and impact evaluation plan, strategies, sampling design and instruments. Train master trainers and field staff for spot-check activities and spot-check surveys. Perform statistical analysis for the monitoring and evaluation and Impact evaluation.
5. Knowledge towards Harm Reduction and Nicotine among Public Health Practitioners in Pakistan Client: ARI Coverage: Islamabad, Rawalpindi Date: Feb -2022 to Jan 2023 Role: Senior Researcher Responsibilities: Developed survey questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Perform quantitative and qualitative data analysis and report writing. 6. Baseline Survey for Global Climate Fund (GCF) Project “Transforming the Indus Basin with Climate Resilient Agriculture and Water Management” Client: FAO/Momentum Ventures Coverage: Dera Ghazi Khan, Khanewal, Lodhran, Multan, Muzaffargarh, Badin, Sanghar, and Umerkot Date: April 2022- July 2022 Role: Team Leader Responsibilities: Manage and control project activities, developed survey tools and questionnaires, survey-sampling design. Train enumerators for survey activities. Manage quantitative and qualitative data analysis and report writing and editing.
7. Assessing the Knowledge, Attitude and Practices of Cigarette Smokers and Use of Alternative Nicotine Delivery Systems in Pakistan: A Cross-Sectional Study Client: ARI Coverage: Islamabad, Lahore, Multan, Muzaffargarh, Hyderabad, Karachi, Nawabshah, Peshawar, Swabi, Quetta, Qila Abdullah, Bagh, Ghanche Date: April 2020- Feb 2020 Role assigned: Senior Research Analyst Responsibilities performed: Developed KAP questionnaire, survey implementation plan and sampling design. Train enumerators for survey activities. Performed quantitative and qualitative data analysis and report writing. 8. Rapid Assessment of Smoking and Vaping in Pakistan during COVID-19 Lockdown Client: ARI Coverage: Islamabad, Rawalpindi, Lahore, Faisalabad, Sialkot, Multan, Peshawar, Abbottabad, Quetta, Karachi, and Hyderabad Date: Jan 2020- May 2020 Role assigned: Senior Research Analyst Responsibilities performed: Developed semi-structure questionnaire, survey implementation plan and sampling design. Train enumerators for telephonic survey activities. Performed quantitative and qualitative data analysis and report writing. 9. Knowledge, Attitude and Practice (KAP) Study on Vapers in Pakistan Client: ARI Coverage: Islamabad, Rawalpindi, Sialkot, Lahore, Faisalabad, Multan, Hyderabad, Karachi, Peshawar, Abbottabad, and Quetta Date: July 2019- December 2019 Role assigned: Senior Research Analyst Responsibilities performed: Developed KAP questionnaire, survey implementation plan and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed quantitative and qualitative data analysis and report writing. 10. Barriers to Smoking Cessation in Marginalized Communities and the Use of Harm reduction Product in Islamabad, Pakistan Client: ARI Coverage: Marginalized Area of Islamabad Date: Jan 2019- Jun 2019 Role assigned: Senior Research Analyst Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative data analysis and report writing. 11. Socio-economic Profile of Out-of-School Children 5-16 Years of Age in Pakistan: A Preliminary Analysis Client: Pakistan Coalition for Education Coverage: Secondary Research from 2014-2019 across Pakistan Date: Jan- June 2020 Role assigned: Research Analyst Responsibilities performed: Performed Secondary data analysis and statistical report writing. 12. Rapid Assessment of Psychological Effect of COVID-19 & School closure on Children Client: Pakistan Coalition for Education Coverage: Islamabad, Rawalpindi, Karachi, Peshawar, Quetta, Muzaffarabad, and Gilgit Date: Jun 2020- December 2020 Role assigned: Research Analyst Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative and quantitative data analysis. 13. Third Party Verification of Waseela-e-Rozgar Liabilities Client: BISP Coverage: 77 districts (20 Service providers and 213 training institutes) Date: Dec 2019- April 2020 Role assigned: Senior Economic Analyst Responsibilities performed: Developed questionnaire, survey strategies and sampling design. Train enumerators, supervisors and field staff for M&E activities and spot check. Performed qualitative data analysis and report writing. 14. Multi-topic Targeting Performance Survey Client: BISP/The World Bank Coverage: 4 districts Date: March –June 2017 Role assigned: Economic Analyst Responsibilities performed: Developed survey instruments, developed sample design, delivered data collection training, supervised district teams. Performed data analysis 15. Youth Workforce Development Project (YWFD) Evaluation Client: MSI/USAID: Coverage: Karachi Dates: Aug 2016-Oct 2016 Role assigned: Economic Analyst Responsibilities performed: Data Management and Analysis 16. Humqadam School Construction & Rehabilitation Programme: Baseline Assessment Client: IMC Worldwide: Coverage: 7 districts Dates: Apr 2016-Jul 2016 Role assigned: Economic Analyst Responsibilities performed: Data Management and Analysis 17. Operational Review of the Waseela-e-Taleem Programme Client: BISP/ The World Bank Coverage: 13 districts Dates: April 2013- June 2017 Role assigned: Economic Analyst Responsibilities performed: Delivered data collection training to enumerators and supervisors, developed sample design, developed structural questionnaire, carried out data analysis 18. Monitoring of the National Household Survey of Social Protection and Cohesion in Pakistan Client: The World Bank Coverage: 90 cluster across Pakistan Date: Nov 2015 – March 2016 Role assigned: Economic Analyst, Field Supervisor Responsibilities performed: Monitoring of data collection activities, secondary sampling unit selection, carried out data cleaning, coding and analysis 19. Pakistan Household Skills Measurement Survey (Wave II & III) Client: The World Bank Coverage: 17 districts Date 2014 –2015 Role assigned: Research Assistant Responsibilities performed: Data cleaning, coding, and analysis 20. Punjab Skills Development Fund Furniture Manufacturing Sector Skill Study Client: Punjab Skill Development Fund (Govt. Of Pakistan) Coverage: 4 districts Date: April – November 2015 Role assigned: Research Assistant Responsibilities performed: Sampling design, data cleaning, coding, and data analysis 21. The Agriculture Drip Irrigation Discrete Choice Experiment Survey Client: IFPRI Coverage: 4 districts Date: Jan 2015-Jun 2015 Role assigned: Research Assistant Responsibilities performed: Data Management, data cleaning, coding, and data analysis
22. Baseline Survey for Income Generation project Phase I Client: World Vision Coverage: 5 districts Date: 2015 Role assigned: Research Assistant Responsibilities performed: Sampling design, data cleaning, coding, and analysis 23. Pakistan Panel Rural Household Survey Client: IFPRI Coverage: 19 districts Date: 2012 – 2015 Role assigned: Research Assistant Responsibilities performed: Data cleaning, coding, and analysis 24. Pakistan Agriculture Production Survey Client: IFPRI Coverage: 19 districts Date: 2014 – 2015 Role assigned: Research Assistant, Field supervisor Responsibilities performed: Data cleaning, coding, and analysis
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